• Conditional persistence and tolerance characterize endoparasite–colonial host interactions

      Fontes, I; Hartikainen, H; Taylor, Nick GH; Okamura, B (2017-07)
    • Malacosporean myxozoans exploit a diversity of fish hosts

      Naldoni, Juliana; Adriano, EA; hartigan, ashlie; Sayer, C; Okamura, B (Cambridge University Press (CUP), 2019-06)
      Myxozoans are widespread and common endoparasites of fish with complex life cycles, infecting vertebrate and invertebrate hosts. There are two classes: Myxosporea and Malacosporea. To date about 2500 myxosporean species have been described. By comparison, there are only five described malacosporean species. Malacosporean development in the invertebrate hosts (freshwater bryozoans) has been relatively well studied but is poorly known in fish hosts. Our aim was to investigate the presence and development of malacosporeans infecting a diversity of fish from Brazil, Europe and the USA. We examined kidney from 256 fish belonging variously to the Salmonidae, Cyprinidae, Nemacheilidae, Esocidae, Percidae, Polyodontidae, Serrasalmidae, Cichlidae and Pimelodidae. Malacosporean infections were detected and identified by polymerase chain reaction and small subunit ribosomal DNA sequencing, and the presence of sporogonic stages was evaluated by ultrastructural examination. We found five malacosporean infections in populations of seven European fish species (brown trout, rainbow trout, white fish, dace, roach, gudgeon and stone loach). Ultrastructural analyses revealed sporogonic stages in kidney tubules of three fish species (brown trout, roach and stone loach), providing evidence that fish belonging to at least three families are true hosts. These results expand the range of fish hosts exploited by malacosporeans to complete their life cycle.
    • Occurrence of Schistosoma bovis on Pemba Island, Zanzibar: implications for urogenital schistosomiasis transmission monitoring - CORRIGENDUM (vol 145, pg 1727, 2018)

      Pennance, T; Ame, SM; Amour, AK; Suleiman, KR; Allan, F; Rollinson, D; Webster, BL (Cambridge University Press, 2018-11)
    • Vertical transmission of Tetracapsuloides bryosalmonae (Myxozoa), the causative agent of salmonid proliferative kidney disease

      Abd-Elfattah, Ahmed; Fontes, I; Kumar, Gokhlesh; Soliman, Hatem; Hartikainen, H; Okamura, B; El-Matbouli, Mansour (2014-04)
    • Whole genome amplification and exome sequencing of archived schistosome miracidia

      Le Clec'h, W; Chevalier, FD; McDew-White, M; Allan, F; Webster, BL; Gouvras, AN; Kinunghi, S; Tchuem Tchuenté, L-A; Garba, A; Mohammed, KA; et al. (Cambridge University Press, 2018-05-28)
      Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schistosomiasis Collection at the Natural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.