Does Micro-CT scanning damage DNA in museum specimens?

Hall, A; Sherlock, E; Sykes, D

dc.contributor.author	Hall, A
dc.contributor.author	Sherlock, E
dc.contributor.author	Sykes, D
dc.date.accessioned	2016-06-10T10:06:42Z
dc.date.available	2016-06-10T10:06:42Z
dc.date.issued	2015	en_US
dc.date.submitted	2016-04-25
dc.identifier.citation	Does Micro-CT scanning damage DNA in museum specimens? Hall, A., Sherlock, E., Sykes, D. Journal of Natural Science Collections, Volume 2, pages 22 - 29	en
dc.identifier.uri	http://hdl.handle.net/10141/612488
dc.description.abstract	X-ray micro-computed tomography and DNA sequencing are useful and increasingly common tools in taxonomy and collections research. Whilst the benefits of each method are continually evaluated and debated individually, how the methods impact each other requires more attention. We compared DNA fragment length and the barcode sequence CO1 in samples throughout a CT-scanning protocol, for a range of X-ray exposures and energies. We found no evidence of DNA damage, but advise caution when using precious or archival material, highlighting the need for further investigations and considering potential areas for research.
dc.language.iso	English	en_US
dc.publisher	Natural Science Collections Association	en_US
dc.relation.url	http://www.natsca.org/article/2075	en
dc.relation.url	http://www.natsca.org/sites/default/files/publications/JoNSC%20Vol2-3.PDF	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.title	Does Micro-CT scanning damage DNA in museum specimens?	en_US
dc.type	Journal Article
dc.identifier.journal	Journal of Natural Science Collections	en_US
dc.conference.location	UK	en_US
dc.identifier.volume	2	en_US
dc.identifier.startpage	22 - 28	en_US
pubs.organisational-group	/Natural History Museum
pubs.organisational-group	/Natural History Museum/Science Group
pubs.organisational-group	/Natural History Museum/Science Group/Core Research Laboratories
pubs.organisational-group	/Natural History Museum/Science Group/Core Research Laboratories/Imaging and Analysis Centre (IAC)
pubs.organisational-group	/Natural History Museum/Science Group/Core Research Laboratories/Molecular Biology Laboratories
pubs.organisational-group	/Natural History Museum/Science Group/Functional groups
pubs.organisational-group	/Natural History Museum/Science Group/Functional groups/Facilities Support
pubs.organisational-group	/Natural History Museum/Science Group/Functional groups/Other Support
pubs.organisational-group	/Natural History Museum/Science Group/Life Sciences
dc.embargo	Not known	en_US
elements.import.author	Hall, A	en_US
elements.import.author	Sherlock, E	en_US
elements.import.author	Sykes, D	en_US
dc.description.nhm	NatSCA supports open access publication as part of its mission is to promote and support natural science collections. NatSCA uses the Creative Commons Attribution License (CCAL) http://creativecommons.org/licenses/by/2.5/ for all works we publish. Under CCAL authors retain ownership of the copyright for their article, but authors allow anyone to download, reuse, reprint, modify, distribute, and/or copy articles in NatSCA publications, so long as the original authors and source are cited. The attached file is the published version of the article.
dc.description.nhm	NHM Repository
dc.subject.nhm	Micro-computed tomography; X-ray microtomography; DNA fragmentation; PCR; Sanger sequencing; Barcoding; Lumbricus terrestris; Oligochaeta
refterms.dateFOA	2019-03-01T08:37:09Z
html.description.abstract	X-ray micro-computed tomography and DNA sequencing are useful and increasingly common tools in taxonomy and collections research. Whilst the benefits of each method are continually evaluated and debated individually, how the methods impact each other requires more attention. We compared DNA fragment length and the barcode sequence CO1 in samples throughout a CT-scanning protocol, for a range of X-ray exposures and energies. We found no evidence of DNA damage, but advise caution when using precious or archival material, highlighting the need for further investigations and considering potential areas for research.