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dc.contributor.authorGlenn, TC
dc.contributor.authorLance, SL
dc.contributor.authorMcKee, AM
dc.contributor.authorWebster, BL
dc.contributor.authorEmery, AM
dc.contributor.authorZerlotini, A
dc.contributor.authorOliveira, G
dc.contributor.authorRollinson, D
dc.contributor.authorFaircloth, BC
dc.identifier.citationGlenn et al.: Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database. Parasites & Vectors 2013 6:300. doi:10.1186/1756-3305-6-300en_US
dc.description.abstractBackground Urogenital schistosomiasis caused by Schistosoma haematobium is widely distributed across Africa and is increasingly being targeted for control. Genome sequences and population genetic parameters can give insight into the potential for population- or species-level drug resistance. Microsatellite DNA loci are genetic markers in wide use by Schistosoma researchers, but there are few primers available for S. haematobium. Methods We sequenced 1,058,114 random DNA fragments from clonal cercariae collected from a snail infected with a single Schistosoma haematobium miracidium. We assembled and aligned the S. haematobium sequences to the genomes of S. mansoni and S. japonicum, identifying microsatellite DNA loci across all three species and designing primers to amplify the loci in S. haematobium. To validate our primers, we screened 32 randomly selected primer pairs with population samples of S. haematobium. Results We designed >13,790 primer pairs to amplify unique microsatellite loci in S. haematobium, (available at The three Schistosoma genomes contained similar overall frequencies of microsatellites, but the frequency and length distributions of specific motifs differed among species. We identified 15 primer pairs that amplified consistently and were easily scored. We genotyped these 15 loci in S. haematobium individuals from six locations: Zanzibar had the highest levels of diversity; Malawi, Mauritius, Nigeria, and Senegal were nearly as diverse; but the sample from South Africa was much less diverse. Conclusions About half of the primers in the database of Schistosoma haematobium microsatellite DNA loci should yield amplifiable and easily scored polymorphic markers, thus providing thousands of potential markers. Sequence conservation among S. haematobium, S. japonicum, and S. mansoni is relatively high, thus it should now be possible to identify markers that are universal among Schistosoma species (i.e., using DNA sequences conserved among species), as well as other markers that are specific to species or species-groups (i.e., using DNA sequences that differ among species). Full genome-sequencing of additional species and specimens of S. haematobium, S. japonicum, and S. mansoni is desirable to better characterize differences within and among these species, to develop additional genetic markers, and to examine genes as well as conserved non-coding elements associated with drug resistance.en_US
dc.publisherSpringer Natureen_US
dc.titleSignificant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide databaseen_US
dc.typeJournal Articleen_US
dc.identifier.journalParasites & Vectorsen_US
dc.identifier.startpage300 - 300en_US
dc.internal.reviewer-noteOpen access - approve asap.en
pubs.organisational-group/Natural History Museum
pubs.organisational-group/Natural History Museum/Science Group
pubs.organisational-group/Natural History Museum/Science Group/Functional groups
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research/LS Research
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors/Parasites and Vectors - Research
dc.embargoNot knownen_US
elements.import.authorGlenn, TCen_US
elements.import.authorLance, SLen_US
elements.import.authorMcKee, AMen_US
elements.import.authorWebster, BLen_US
elements.import.authorEmery, AMen_US
elements.import.authorZerlotini, Aen_US
elements.import.authorOliveira, Gen_US
elements.import.authorRollinson, Den_US
elements.import.authorFaircloth, BCen_US
dc.description.nhm© 2013 Glenn et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The attached file is the published version of the article.en_US
dc.subject.nhmPrimer databaseen_US
dc.subject.nhmSchistosoma haematobiumen_US
dc.subject.nhmUrogenital schistosomiasisen_US

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