Trimitomics: An efficient pipeline for mitochondrial assembly from transcriptomic reads in nonmodel species
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Authors
Plese, BrunaRossi, Maria Eleonora
Kenny, Nathan James
Taboada, Sergi
Koutsouveli, Vasiliki
Riesgo, Ana
Issue date
2019-05-09Submitted date
2019-08-08Subject Terms
assemblyinvertebrates
mitochondrial genome
transcriptomics
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Show full item recordAbstract
Mitochondrial resources are of known utility to many fields of phylogenetic, population and molecular biology. Their combination of faster and slower‐evolving regions and high copy number enables them to be used in many situations where other loci are unsuitable, with degraded samples and after recent speciation events.The advent of next‐generation sequencing technologies (and notably the Illumina platform) has led to an explosion in the number of samples that can be studied at transcriptomic level, at relatively low cost. Here we describe a robust pipeline for the recovery of mitochondrial genomes from these RNA‐sequencing resources. This pipeline can be used on sequencing of a variety of depths, and reliably recovers the protein coding and ribosomal gene complements of mitochondria from almost any transcriptomic sequencing experiment. The complete sequence of the mitochondrial genome can also be recovered when sequencing is performed in sufficient depth. We show the efficacy of our pipeline using data from eight nonmodel invertebrates of six disparate phyla. Interestingly, among our poriferan data, where microbiological symbionts are known empirically to make mitochondrial assembly difficult, this pipeline proved especially useful. Our pipeline will allow the recovery of mitochondrial data from a variety of previously sequenced samples, and add an additional angle of enquiry to future RNA‐sequencing efforts, simplifying the process of mitochondrial genome assembly for even the most recalcitrant clades and adding these data to the scientific record for a range of future uses.Citation
Plese, B, Rossi, ME, Kenny, NJ, Taboada, S, Koutsouveli, V, Riesgo, A. Trimitomics: An efficient pipeline for mitochondrial assembly from transcriptomic reads in nonmodel species. Mol Ecol Resour. 2019; 00: 1– 10. https://doi.org/10.1111/1755-0998.13033Publisher
WileyJournal
Molecular Ecology ResourcesType
Journal ArticleItem Description
© 2019 John Wiley & Sons Ltd This is the pre-peer reviewed version of the following article: Plese, B, Rossi, ME, Kenny, NJ, Taboada, S, Koutsouveli, V, Riesgo, A. Trimitomics: An efficient pipeline for mitochondrial assembly from transcriptomic reads in nonmodel species. Mol Ecol Resour. 2019; 00: 1– 10. https://doi.org/10.1111/1755-0998.13033, which has been published in final form at https://doi.org/10.1111/1755-0998.13033. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."NHM Repository
EISSN
1755-0998ae974a485f413a2113503eed53cd6c53
10.1111/1755-0998.13033
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