Molecular characterization and distribution of Schistosoma cercariae collected from naturally infected bulinid snails in northern and central Côte d’Ivoire
Name:
s13071-019-3381-3.pdf
Size:
2.516Mb
Format:
PDF
Description:
Published/publisher’s pdf
Name:
13071_2019_3381_MOESM1_ESM.xlsx
Size:
21.62Kb
Format:
Microsoft
Excel 2007
Description:
Supporting information
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to
this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Tian-Bi, Y-NTWebster, BL
Konan, CK
Allan, F
Diakité, NR
Ouattara, M
Salia, D
Koné, A
Kakou, AK
Rabone, M
Coulibaly, JT
Knopp, S
Meïté, A
Utzinger, J
N’Goran, EK
Rollinson, D
Issue date
2019-03-19Submitted date
2019-10-30Subject Terms
Bulinus forskaliiBulinus globosus
Bulinus truncatus
Côte d’Ivoire
Molecular markers
Schistosoma bovis
Schistosoma haematobium
Schistosome hybrids
Metadata
Show full item recordAbstract
Accurate identification of schistosome species infecting intermediate host snails is important for understanding parasite transmission, schistosomiasis control and elimination. Cercariae emerging from infected snails cannot be precisely identified morphologically to the species level. We used molecular tools to clarify the distribution of the Schistosoma haematobium group species infecting bulinid snails in a large part of Côte d’Ivoire and confirmed the presence of interspecific hybrid schistosomes. Methods Between June 2016 and March 2017, Bulinus snails were sampled in 164 human-water contact sites from 22 villages of the northern and central parts of Côte d’Ivoire. Multi-locus genetic analysis (mitochondrial cox1 and nuclear ITS) was performed on individual schistosome cercariae shed from snails, in the morning and in the afternoon, for species and hybrid identification. Results Overall, 1923 Bulinus truncatus, 255 Bulinus globosus and 1424 Bulinus forskalii were obtained. Among 2417 Bulinus screened, 25 specimens (18 B. truncatus and seven B. globosus) shed schistosomes, with up to 14% infection prevalence per site and time point. Globally, infection rates per time point ranged between 0.6 and 4%. Schistosoma bovis, S. haematobium and S. bovis × S. haematobium hybrids infected 0.5%, 0.2% and 0.4% of the snails screened, respectively. Schistosoma bovis and hybrids were more prevalent in B. truncatus, whereas S. haematobium and hybrid infections were more prevalent in B. globosus. Schistosoma bovis-infected Bulinus were predominantly found in northern sites, while S. haematobium and hybrid infected snails were mainly found in central parts of Côte d’Ivoire. Conclusions The data highlight the necessity of using molecular tools to identify and understand which schistosome species are transmitted by specific intermediate host snails. The study deepens our understanding of the epidemiology and transmission dynamics of S. haematobium and S. bovis in Côte d’Ivoire and provides the first conclusive evidence for the transmission of S. haematobium × S. bovis hybrids in this West African country. Trial registration ISRCTN, ISRCTN10926858. Registered 21 December 2016; retrospectively registered (see: http://www.isrctn.com/ISRCTN10926858)Citation
Tian-Bi, Y.T., Webster, B., Konan, C.K. et al. Molecular characterization and distribution of Schistosoma cercariae collected from naturally infected bulinid snails in northern and central Côte d’Ivoire. Parasites Vectors 12, 117 (2019) doi:10.1186/s13071-019-3381-3Publisher
Springer NatureJournal
Parasites & VectorsType
Journal ArticleItem Description
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The attached file is the published pdf.NHM Repository
EISSN
1756-3305ae974a485f413a2113503eed53cd6c53
10.1186/s13071-019-3381-3
Scopus Count
Collections