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dc.contributor.authorSumner-Rooney, L
dc.contributor.authorKenny, Nathan
dc.contributor.authorAhmed, F
dc.contributor.authorWilliams, ST
dc.date.accessioned2019-11-21T16:24:05Z
dc.date.available2019-11-21T16:24:05Z
dc.date.issued2019-10-28
dc.date.submitted2019-11-18
dc.identifier.citationSumner-Rooney, L., Kenny, N.J., Ahmed, F. et al. The utility of micro-computed tomography for the non-destructive study of eye microstructure in snails. Sci Rep 9, 15411 (2019) doi:10.1038/s41598-019-51909-zen_US
dc.identifier.doi10.1038/s41598-019-51909-z
dc.identifier.urihttp://hdl.handle.net/10141/622590
dc.description.abstractMolluscan eyes exhibit an enormous range of morphological variation, ranging from tiny pigment-cup eyes in limpets, compound eyes in ark clams and pinhole eyes in Nautilus, through to concave mirror eyes in scallops and the large camera-type eyes of the more derived cephalopods. Here we assess the potential of non-destructive micro-computed tomography (µ-CT) for investigating the anatomy of molluscan eyes in three species of the family Solariellidae, a group of small, deep-sea gastropods. We compare our results directly with those from traditional histological methods applied to the same specimens, and show not only that eye microstructure can be visualised in sufficient detail for meaningful comparison even in very small animals, but also that μ-CT can provide additional insight into gross neuroanatomy without damaging rare and precious specimens. Data from μ-CT scans also show that neurological innervation of eyes is reduced in dark-adapted snails when compared with the innervation of cephalic tentacles, which are involved in mechanoreception and possibly chemoreception. Molecular tests also show that the use of µ-CT and phosphotungstic acid stain do not prevent successful downstream DNA extraction, PCR amplification or sequencing. The use of µ-CT methods is therefore highly recommended for the investigation of difficult-to-collect or unique specimens.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.rightsopenAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe utility of micro-computed tomography for the non-destructive study of eye microstructure in snailsen_US
dc.typeJournal Articleen_US
dc.identifier.eissn2045-2322
dc.identifier.journalScientific Reportsen_US
dc.identifier.volume9en_US
dc.identifier.issue1en_US
pubs.organisational-group/Natural History Museum
pubs.organisational-group/Natural History Museum/Science Group
pubs.organisational-group/Natural History Museum/Science Group/Functional groups
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Other Support
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research/LS Research
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences
dc.embargoNot knownen_US
elements.import.authorSumner-Rooney, Len_US
elements.import.authorKenny, NJen_US
elements.import.authorAhmed, Fen_US
elements.import.authorWilliams, STen_US
dc.description.nhmOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The attached file is the published pdf.en_US
dc.subject.nhmAnimal physiologyen_US
dc.subject.nhmEvolutionen_US
dc.subject.nhmX-ray tomographyen_US
refterms.dateFOA2019-11-21T16:24:07Z


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