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dc.contributor.authorPAPAIAKOVOU, MARINA
dc.contributor.authorWright, J
dc.contributor.authorPilotte, N
dc.contributor.authorChooneea, D
dc.contributor.authorSchär, F
dc.contributor.authorTruscott, JE
dc.contributor.authorDunn, JC
dc.contributor.authorGardiner, I
dc.contributor.authorWalson, JL
dc.contributor.authorWilliams, SA
dc.contributor.authorLittlewood, T
dc.date.accessioned2019-12-12T12:36:53Z
dc.date.available2019-12-12T12:36:53Z
dc.date.issued2019-09-16
dc.date.submitted2019-12-03
dc.identifier.citationPapaiakovou, M., Wright, J., Pilotte, N. et al. Pooling as a strategy for the timely diagnosis of soil-transmitted helminths in stool: value and reproducibility. Parasites Vectors 12, 443 (2019) doi:10.1186/s13071-019-3693-3en_US
dc.identifier.doi10.1186/s13071-019-3693-3
dc.identifier.urihttp://hdl.handle.net/10141/622593
dc.description.abstractBackground The strategy of pooling stool specimens has been extensively used in the field of parasitology in order to facilitate the screening of large numbers of samples whilst minimizing the prohibitive cost of single sample analysis. The aim of this study was to develop a standardized reproducible pooling protocol for stool samples, validated between two different laboratories, without jeopardizing the sensitivity of the quantitative polymerase chain reaction (qPCR) assays employed for the detection of soil-transmitted helminths (STHs). Two distinct experimental phases were recruited. First, the sensitivity and specificity of the established protocol was assessed by real-time PCR for each one of the STHs. Secondly, agreement and reproducibility of the protocol between the two different laboratories were tested. The need for multiple stool sampling to avoid false negative results was also assessed. Finally, a cost exercise was conducted which included labour cost in low- and high-wage settings, consumable cost, prevalence of a single STH species, and a simple distribution pattern of the positive samples in pools to estimate time and money savings suggested by the strategy. Results The sensitivity of the pooling method was variable among the STH species but consistent between the two laboratories. Estimates of specificity indicate a ‘pooling approach’ can yield a low frequency of ‘missed’ infections. There were no significant differences regarding the execution of the protocol and the subsequent STH detection between the two laboratories, which suggests in most cases the protocol is reproducible by adequately trained staff. Finally, given the high degree of agreement, there appears to be little or no need for multiple sampling of either individuals or pools. Conclusions Our results suggest that the pooling protocol developed herein is a robust and efficient strategy for the detection of STHs in ‘pools-of-five’. There is notable complexity of the pool preparation to ensure even distribution of helminth DNA throughout. Therefore, at a given setting, cost of labour among other logistical and epidemiological factors, is the more concerning and determining factor when choosing pooling strategies, rather than losing sensitivity and/or specificity of the molecular assay or the method.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.rightsopenAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titlePooling as a strategy for the timely diagnosis of soil-transmitted helminths in stool: value and reproducibilityen_US
dc.typeJournal Articleen_US
dc.identifier.eissn1756-3305
dc.identifier.journalParasites & Vectorsen_US
dc.identifier.volume12en_US
dc.identifier.issue1en_US
pubs.organisational-group/Natural History Museum
pubs.organisational-group/Natural History Museum/Access control
pubs.organisational-group/Natural History Museum/Access control/Management LS
pubs.organisational-group/Natural History Museum/Science Group
pubs.organisational-group/Natural History Museum/Science Group/Functional groups
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Research/LS Research
pubs.organisational-group/Natural History Museum/Science Group/Initiatives
pubs.organisational-group/Natural History Museum/Science Group/Initiatives/Natural Resources and Hazards
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors/Parasites and Vectors - Research
pubs.organisational-group/Natural History Museum/Science Group/Science Directorate
pubs.organisational-group/Natural History Museum/Science Group/Science Directorate/Science Directorate
dc.embargoNot knownen_US
elements.import.authorPapaiakovou, Men_US
elements.import.authorWright, Jen_US
elements.import.authorPilotte, Nen_US
elements.import.authorChooneea, Den_US
elements.import.authorSchär, Fen_US
elements.import.authorTruscott, JEen_US
elements.import.authorDunn, JCen_US
elements.import.authorGardiner, Ien_US
elements.import.authorWalson, JLen_US
elements.import.authorWilliams, SAen_US
elements.import.authorLittlewood, DTJen_US
dc.description.nhmOpen Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.description.nhmNHM Repository
dc.subject.nhmBreakpoint of transmissionen_US
dc.subject.nhmCost analysis of poolingen_US
dc.subject.nhmPoolingen_US
dc.subject.nhmSoil-transmitted helminthsen_US
dc.subject.nhmStool samplesen_US
dc.subject.nhmqPCR-based diagnosticsen_US
refterms.dateFOA2019-12-12T12:36:54Z


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