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dc.contributor.authorJex, AR
dc.contributor.authorHall, RS
dc.contributor.authorLittlewood, T
dc.contributor.authorGasser, RB
dc.identifier.citationAaron R. Jex, Ross S. Hall, D. Timothy J. Littlewood, Robin B. Gasser, An integrated pipeline for next-generation sequencing and annotation of mitochondrial genomes, Nucleic Acids Research, Volume 38, Issue 2, 1 January 2010, Pages 522–533,
dc.description.abstractMitochondrial (mt) genomics represents an understudied but important field of molecular biology. Increasingly, mt dysfunction is being linked to a range of human diseases, including neurodegenerative disorders, diabetes and impairment of childhood development. In addition, mt genomes provide important markers for systematic, evolutionary and population genetic studies. Some technological limitations have prevented the expanded generation and utilization of mt genomic data for some groups of organisms. These obstacles most acutely impede, but are not limited to, studies requiring the determination of complete mt genomic data from minute amounts of material (e.g. biopsy samples or microscopic organisms). Furthermore, post-sequencing bioinformatic annotation and analyses of mt genomes are time consuming and inefficient. Herein, we describe a high-throughput sequencing and bioinformatic pipeline for mt genomics, which will have implications for the annotation and analysis of other organellar (e.g. plastid or apicoplast genomes) and virus genomes as well as long, contiguous regions in nuclear genomes. We utilize this pipeline to sequence and annotate the complete mt genomes of 12 species of parasitic nematode (order Strongylida) simultaneously, each from an individual organism. These mt genomic data provide a rich source of markers for studies of the systematics and population genetics of a group of socioeconomically important pathogens of humans and other animals.en_US
dc.publisherOxford University Press (OUP)en_US
dc.titleAn integrated pipeline for next-generation sequencing and annotation of mitochondrial genomesen_US
dc.typeJournal Articleen_US
dc.identifier.journalNucleic Acids Researchen_US
dc.identifier.startpage522 - 533en_US
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dc.embargoNot knownen_US
elements.import.authorJex, ARen_US
elements.import.authorHall, RSen_US
elements.import.authorLittlewood, DTJen_US
elements.import.authorGasser, RBen_US
dc.description.nhm© The Author(s) 2009. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The attached file is the published pdf.en_US
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dc.subject.nhmGene sequencingen_US
dc.subject.nhmHuman parasitesen_US

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