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    High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium

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    Authors
    Zheng, W
    Gao, F
    Warren, A cc
    Issue date
    2015-10-22
    Submitted date
    2015
    Subject Terms
    Axenic cultivation
    ciliates
    fish parasite
    scuticociliatosis
    Uronema marinum
    
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    Abstract
    Uronema marinum is a cosmopolitan marine ciliate. It is a facultative parasite and the main causative agent of outbreaks of scuticociliatosis in aquaculture fish. This study reports a method for the axenic cultivation of U. marinum in high densities in an artificial medium comprising proteose peptone, glucose and yeast extract powder as its basic components. The absence of bacteria in the cultures was confirmed by fluorescence microscopy of DAPI-stained samples and the failure to recover bacterial SSU-rDNA using standard PCR methods. Using this axenic medium, a maximum cell density of 420,000 ciliate cells/ml was achieved, which is significantly higher than in cultures using living bacteria as food or in other axenic media reported previously. This method for high-density axenic cultivation of U. marinum should facilitate future research on this economically important facultative fish parasite.
    Citation
    ZHENG, Weibo; GAO, Feng; WARREN, Alan. High-Density Cultivation of the Marine Ciliate Uronema marinum (Ciliophora, Oligohymenophorea) in Axenic Medium. Acta Protozoologica, 2015, Volume 54 Issue 4, p. 325-330, pa. 2015. ISSN 1689-0027
    Journal
    Acta Protozoologica
    URI
    http://hdl.handle.net/10141/622678
    DOI
    10.4467/16890027AP.15.027.3541
    Type
    Journal Article
    Item Description
    Open access journal. The editor declares the online version to be the original one. The magazine is shown on-line continuously here: http://www.ejournals.eu/Acta-Protozoologica/
    ISSN
    0065-1583
    EISSN
    1689-0027
    ae974a485f413a2113503eed53cd6c53
    10.4467/16890027AP.15.027.3541
    Scopus Count
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    Life sciences

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