Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda)
dc.contributor.author | Jex, AR | |
dc.contributor.author | Hu, M | |
dc.contributor.author | Littlewood, T | |
dc.contributor.author | Waeschenbach, A | |
dc.contributor.author | Gasser, RB | |
dc.date.accessioned | 2020-04-15T10:00:58Z | |
dc.date.available | 2020-04-15T10:00:58Z | |
dc.date.issued | 2008-01-11 | |
dc.date.submitted | 2020-04-06 | |
dc.identifier.citation | Jex, A.R., Hu, M., Littlewood, D.T.J. et al. Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda). BMC Genomics 9, 11 (2008). https://doi.org/10.1186/1471-2164-9-11 | en_US |
dc.identifier.issn | 1471-2164 | |
dc.identifier.doi | 10.1186/1471-2164-9-11 | |
dc.identifier.uri | http://hdl.handle.net/10141/622694 | |
dc.description.abstract | Background: Mitochondrial (mt) genomes represent a rich source of molecular markers for a range of applications, including population genetics, systematics, epidemiology and ecology. In the present study, we used 454 technology (or the GS20, massively parallel picolitre reactor platform) to determine the complete mt genome of Haemonchus contortus (Nematoda: Trichostrongylidae), a parasite of substantial agricultural, veterinary and economic significance. We validate this approach by comparison with mt sequences from publicly available expressed sequence tag (EST) and genomic survey sequence (GSS) data sets. Results: The complete mt genome of Haemonchus contortus was sequenced directly from longPCR amplified template utilizing genomic DNA (~20–40 ng) from a single adult male using 454 technology. A single contig was assembled and compared against mt sequences mined from publicly available EST (NemBLAST) and GSS datasets. The comparison demonstrated that the 454 technology platform is reliable for the sequencing of AT-rich mt genomes from nematodes. The mt genome sequenced for Haemonchus contortus was 14,055 bp in length and was highly AT-rich (78.1%). In accordance with other chromadorean nematodes studied to date, the mt genome of H. contortus contained 36 genes (12 protein coding, 22 tRNAs, rrnL and rrnS) and was similar in structure, size and gene arrangement to those characterized previously for members of the Strongylida. Conclusion: The present study demonstrates the utility of 454 technology for the rapid determination of mt genome sequences from tiny amounts of DNA and reveals a wealth of mt genomic data in current databases available for mining. This approach provides a novel platform for high-throughput sequencing of mt genomes from nematodes and other organisms. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.rights | openAccess | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/2.0/ | |
dc.title | Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda) | en_US |
dc.type | Journal Article | en_US |
dc.identifier.journal | BMC Genomics | en_US |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 11 - 11 | en_US |
pubs.organisational-group | /Natural History Museum | |
pubs.organisational-group | /Natural History Museum/Access control | |
pubs.organisational-group | /Natural History Museum/Access control/Management LS | |
pubs.organisational-group | /Natural History Museum/Science Group | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups/Research | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups/Research/LS Research | |
pubs.organisational-group | /Natural History Museum/Science Group/Initiatives | |
pubs.organisational-group | /Natural History Museum/Science Group/Initiatives/Natural Resources and Hazards | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences/Parasites and Vectors | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences/Parasites and Vectors/Parasites and Vectors - Research | |
pubs.organisational-group | /Natural History Museum/Science Group/Science Directorate | |
pubs.organisational-group | /Natural History Museum/Science Group/Science Directorate/Science Directorate | |
dc.embargo | Not known | en_US |
elements.import.author | Jex, AR | en_US |
elements.import.author | Hu, M | en_US |
elements.import.author | Littlewood, DTJ | en_US |
elements.import.author | Waeschenbach, A | en_US |
elements.import.author | Gasser, RB | en_US |
dc.description.nhm | © 2008 Jex et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
dc.description.nhm | NHM Repository | |
dc.subject.nhm | Genomic Survey Sequence | en_US |
dc.subject.nhm | Haemonchus Contortus | en_US |
dc.subject.nhm | Infer Amino Acid Sequence | en_US |
dc.subject.nhm | Complex Secondary Structure | en_US |
dc.subject.nhm | Strongylid Nematode | en_US |
refterms.dateFOA | 2020-04-15T10:00:59Z |