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dc.contributor.authorJex, AR
dc.contributor.authorHu, M
dc.contributor.authorLittlewood, T
dc.contributor.authorWaeschenbach, A
dc.contributor.authorGasser, RB
dc.date.accessioned2020-04-15T10:00:58Z
dc.date.available2020-04-15T10:00:58Z
dc.date.issued2008-01-11
dc.date.submitted2020-04-06
dc.identifier.citationJex, A.R., Hu, M., Littlewood, D.T.J. et al. Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda). BMC Genomics 9, 11 (2008). https://doi.org/10.1186/1471-2164-9-11en_US
dc.identifier.issn1471-2164
dc.identifier.doi10.1186/1471-2164-9-11
dc.identifier.urihttp://hdl.handle.net/10141/622694
dc.description.abstractBackground: Mitochondrial (mt) genomes represent a rich source of molecular markers for a range of applications, including population genetics, systematics, epidemiology and ecology. In the present study, we used 454 technology (or the GS20, massively parallel picolitre reactor platform) to determine the complete mt genome of Haemonchus contortus (Nematoda: Trichostrongylidae), a parasite of substantial agricultural, veterinary and economic significance. We validate this approach by comparison with mt sequences from publicly available expressed sequence tag (EST) and genomic survey sequence (GSS) data sets. Results: The complete mt genome of Haemonchus contortus was sequenced directly from longPCR amplified template utilizing genomic DNA (~20–40 ng) from a single adult male using 454 technology. A single contig was assembled and compared against mt sequences mined from publicly available EST (NemBLAST) and GSS datasets. The comparison demonstrated that the 454 technology platform is reliable for the sequencing of AT-rich mt genomes from nematodes. The mt genome sequenced for Haemonchus contortus was 14,055 bp in length and was highly AT-rich (78.1%). In accordance with other chromadorean nematodes studied to date, the mt genome of H. contortus contained 36 genes (12 protein coding, 22 tRNAs, rrnL and rrnS) and was similar in structure, size and gene arrangement to those characterized previously for members of the Strongylida. Conclusion: The present study demonstrates the utility of 454 technology for the rapid determination of mt genome sequences from tiny amounts of DNA and reveals a wealth of mt genomic data in current databases available for mining. This approach provides a novel platform for high-throughput sequencing of mt genomes from nematodes and other organisms.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.titleUsing 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda)en_US
dc.typeJournal Articleen_US
dc.identifier.journalBMC Genomicsen_US
dc.identifier.volume9en_US
dc.identifier.issue1en_US
dc.identifier.startpage11 - 11en_US
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dc.embargoNot knownen_US
elements.import.authorJex, ARen_US
elements.import.authorHu, Men_US
elements.import.authorLittlewood, DTJen_US
elements.import.authorWaeschenbach, Aen_US
elements.import.authorGasser, RBen_US
dc.description.nhm© 2008 Jex et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.description.nhmNHM Repository
dc.subject.nhmGenomic Survey Sequenceen_US
dc.subject.nhmHaemonchus Contortusen_US
dc.subject.nhmInfer Amino Acid Sequenceen_US
dc.subject.nhmComplex Secondary Structureen_US
dc.subject.nhmStrongylid Nematodeen_US
refterms.dateFOA2020-04-15T10:00:59Z


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