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dc.contributor.authorDunn, JC
dc.contributor.authorPAPAIAKOVOU, MARINA
dc.contributor.authorHan, KT
dc.contributor.authorChooneea, D
dc.contributor.authorBettis, AA
dc.contributor.authorWyine, NY
dc.contributor.authorLwin, AMM
dc.contributor.authorMaung, NS
dc.contributor.authorMisra, Raju
dc.contributor.authorLittlewood, T
dc.contributor.authorAnderson, RM
dc.date.accessioned2020-06-29T09:31:03Z
dc.date.available2020-06-29T09:31:03Z
dc.date.issued2020-06-24
dc.date.submitted2020-06-27
dc.identifier.citationDunn, J.C., Papaiakovou, M., Han, K.T. et al. The increased sensitivity of qPCR in comparison to Kato-Katz is required for the accurate assessment of the prevalence of soil-transmitted helminth infection in settings that have received multiple rounds of mass drug administration. Parasites Vectors 13, 324 (2020). https://doi.org/10.1186/s13071-020-04197-wen_US
dc.identifier.doi10.1186/s13071-020-04197-w
dc.identifier.urihttp://hdl.handle.net/10141/622793
dc.description.abstractBackground The most commonly used diagnostic tool for soil-transmitted helminths (STH) is the Kato-Katz (KK) thick smear technique. However, numerous studies have suggested that the sensitivity of KK can be problematic, especially in low prevalence and low intensity settings. An emerging alternative is quantitative polymerase chain reaction (qPCR). Methods In this study, both KK and qPCR were conducted on stool samples from 648 participants in an STH epidemiology study conducted in the delta region of Myanmar in June 2016. Results Prevalence of any STH was 20.68% by KK and 45.06% by qPCR. Prevalence of each individual STH was also higher by qPCR than KK, the biggest difference was for hookworm with an approximately 4-fold increase between the two diagnostic techniques. Prevalence of Ancylostoma ceylanicum, a parasite predominately found in dogs, was 4.63%, indicating that there is the possibility of zoonotic transmission in the study setting. In individuals with moderate to high intensity infections there is evidence for a linear relationship between eggs per gram (EPG) of faeces, derived from KK, and DNA copy number, derived from qPCR which is particularly strong for Ascaris lumbricoides. Conclusions The use of qPCR in low prevalence settings is important to accurately assess the epidemiological situation and plan control strategies for the ‘end game’. However, more work is required to accurately assess STH intensity from qPCR results and to reduce the cost of qPCR so that is widely accessible in STH endemic countries.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04197-wen_US
dc.rightsopenAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe increased sensitivity of qPCR in comparison to Kato-Katz is required for the accurate assessment of the prevalence of soil-transmitted helminth infection in settings that have received multiple rounds of mass drug administrationen_US
dc.typeJournal Articleen_US
dc.identifier.eissn1756-3305
dc.identifier.journalParasites & Vectorsen_US
dc.identifier.volume13en_US
dc.identifier.issue1en_US
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pubs.organisational-group/Natural History Museum/Science Group/Life Sciences
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences/Parasites and Vectors/Parasites and Vectors - Research
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dc.embargoNot knownen_US
elements.import.authorDunn, JCen_US
elements.import.authorPapaiakovou, Men_US
elements.import.authorHan, KTen_US
elements.import.authorChooneea, Den_US
elements.import.authorBettis, AAen_US
elements.import.authorWyine, NYen_US
elements.import.authorLwin, AMMen_US
elements.import.authorMaung, NSen_US
elements.import.authorMisra, Ren_US
elements.import.authorLittlewood, DTJen_US
elements.import.authorAnderson, RMen_US
dc.description.nhmOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.en_US
dc.subject.nhmSoil-transmitted helminthsen_US
dc.subject.nhmKato-Katzen_US
dc.subject.nhmqPCRen_US
dc.subject.nhmDiagnosticsen_US
dc.subject.nhmMonitoring and evaluationen_US
refterms.dateFOA2020-06-29T09:31:04Z


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