Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)
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Issue date
2013-12-03Submitted date
2020-08-17Subject Terms
mitochondrial DNAMonogenea
primer design
ribosomal DNA
Rhabdocoela
turbellarians
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Show full item recordAbstract
Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.Citation
Vanhove M, Tessens B, Schoelinck C, Jondelius U, Littlewood T, Artois T, Huyse T (2013) Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes). ZooKeys 365: 355-379. https://doi.org/10.3897/zookeys.365.5776Publisher
Pensoft PublishersJournal
ZooKeysType
Journal ArticleItem Description
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The attached file is the published pdf.ISSN
1313-2989EISSN
1313-2970ae974a485f413a2113503eed53cd6c53
10.3897/zookeys.365.5776
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