Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol
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Issue date
2012-03-08Subject Terms
Calliphora vicinaForensic entomology
Post-mortem interval
Preservation
Storage temperature
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Show full item recordAbstract
The size of immature blowflies is a common measure to estimate the minimum time between death and the discovery of a corpse, also known as the minimum post-mortem interval. This paper investigates the effects of preservation, in 80% ethanol, on the length and weight of first instar, second instar, feeding third instar, and post-feeding third instar Calliphora vicina larvae, at three different storage temperatures. For each larval stage, the length of larvae was recorded after 0 h, 3 h, 6 h, 9 h, 12 h, 24 h, 72 h, 7 days, 14 days, 30 days, 91 days, 182 days, 273 days, and 365 days of storage in 80% ethanol, at −25°C, 6°C and 24°C. Storage temperature had no statistically significant effect on the change in larval length and weight for all larval stages, but larval length and weight were significantly affected by the duration of preservation for first, second, and feeding third instar larvae, but not for post-feeding larvae. Generally, first and second instar larvae reduced in size over time, while feeding third instar larvae increased slightly in size, and post-feeding larvae did not change in size over time. The length of blowfly larvae preserved in 80% ethanol is not affected by constant storage temperatures between −25°C and +24°C, but we recommend that forensic entomologists should use the models provided to correct for changes in larval length that do become apparent over time.Citation
Richards, C.S., Rowlinson, C.C. & Hall, M.J.R. Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol. Int J Legal Med 127, 231–241 (2013). https://doi.org/10.1007/s00414-012-0683-9Publisher
Springer Science and Business Media LLCType
Journal ArticleItem Description
Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ISSN
0937-9827EISSN
1437-1596ae974a485f413a2113503eed53cd6c53
10.1007/s00414-012-0683-9
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