Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol
dc.contributor.author | Richards, CS | |
dc.contributor.author | Rowlinson, CC | |
dc.contributor.author | Hall, MJR | |
dc.date.accessioned | 2020-09-08T10:43:07Z | |
dc.date.available | 2020-09-08T10:43:07Z | |
dc.date.issued | 2012-03-08 | |
dc.identifier.citation | Richards, C.S., Rowlinson, C.C. & Hall, M.J.R. Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol. Int J Legal Med 127, 231–241 (2013). https://doi.org/10.1007/s00414-012-0683-9 | en_US |
dc.identifier.issn | 0937-9827 | |
dc.identifier.doi | 10.1007/s00414-012-0683-9 | |
dc.identifier.uri | http://hdl.handle.net/10141/622818 | |
dc.description.abstract | The size of immature blowflies is a common measure to estimate the minimum time between death and the discovery of a corpse, also known as the minimum post-mortem interval. This paper investigates the effects of preservation, in 80% ethanol, on the length and weight of first instar, second instar, feeding third instar, and post-feeding third instar Calliphora vicina larvae, at three different storage temperatures. For each larval stage, the length of larvae was recorded after 0 h, 3 h, 6 h, 9 h, 12 h, 24 h, 72 h, 7 days, 14 days, 30 days, 91 days, 182 days, 273 days, and 365 days of storage in 80% ethanol, at −25°C, 6°C and 24°C. Storage temperature had no statistically significant effect on the change in larval length and weight for all larval stages, but larval length and weight were significantly affected by the duration of preservation for first, second, and feeding third instar larvae, but not for post-feeding larvae. Generally, first and second instar larvae reduced in size over time, while feeding third instar larvae increased slightly in size, and post-feeding larvae did not change in size over time. The length of blowfly larvae preserved in 80% ethanol is not affected by constant storage temperatures between −25°C and +24°C, but we recommend that forensic entomologists should use the models provided to correct for changes in larval length that do become apparent over time. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.rights | openAccess | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/2.0/ | |
dc.title | Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol | en_US |
dc.type | Journal Article | en_US |
dc.identifier.eissn | 1437-1596 | |
dc.identifier.journal | International Journal of Legal Medicine | en_US |
dc.identifier.volume | 127 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 231 - 241 | en_US |
pubs.organisational-group | /Natural History Museum | |
pubs.organisational-group | /Natural History Museum/Science Group | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups/Research | |
pubs.organisational-group | /Natural History Museum/Science Group/Functional groups/Research/LS Research | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences/Parasites and Vectors | |
pubs.organisational-group | /Natural History Museum/Science Group/Life Sciences/Parasites and Vectors/Parasites and Vectors - Research | |
dc.embargo | Not known | en_US |
elements.import.author | Richards, CS | en_US |
elements.import.author | Rowlinson, CC | en_US |
elements.import.author | Hall, MJR | en_US |
dc.description.nhm | Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
dc.description.nhm | NHM Repository | |
dc.subject.nhm | Calliphora vicina | en_US |
dc.subject.nhm | Forensic entomology | en_US |
dc.subject.nhm | Post-mortem interval | en_US |
dc.subject.nhm | Preservation | en_US |
dc.subject.nhm | Storage temperature | en_US |
refterms.dateFOA | 2020-09-08T10:43:08Z |