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dc.contributor.authorHupało, K
dc.contributor.authorMajaneva, M
dc.contributor.authorCzachur, MV
dc.contributor.authorSire, L
dc.contributor.authorMarquina, D
dc.contributor.authorLijtmaer, DA
dc.contributor.authorIvanov, V
dc.contributor.authorLeidenberger, S
dc.contributor.authorČiampor, F
dc.contributor.authorČiamporová‐Zaťovičová, Z
dc.contributor.authorMendes, IS
dc.contributor.authorDesiderato, A
dc.contributor.authorTopstad, L
dc.contributor.authorMeganck, K
dc.contributor.authorHariz Z. A., D
dc.contributor.authorKjærstad, G
dc.contributor.authorLin, X
dc.contributor.authorPrice, BW
dc.contributor.authorStevens, M
dc.contributor.authorEkrem, T
dc.contributor.authorDeiner, K
dc.date.accessioned2020-11-02T13:57:49Z
dc.date.available2020-11-02T13:57:49Z
dc.date.issued2020-10-24
dc.date.submitted2020-10-29
dc.identifier.citationHupało, K, Majaneva, M, Czachur, MV, et al. An urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNA. Environmental DNA. 2020; 00: 1– 14. https://doi.org/10.1002/edn3.152en_US
dc.identifier.issn2637-4943
dc.identifier.doi10.1002/edn3.152
dc.identifier.urihttp://hdl.handle.net/10141/622868
dc.description.abstractAs global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people of all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we explore whether sampling eDNA and sequencing it can be used as a means of rapidly surveying urban biodiversity for educational purposes. We sampled 2 × 1 L of water from each of 15 locations in the city of Trondheim, Norway, including a variety of freshwater, marine, and brackish habitats. DNA was extracted, amplified in triplicate targeting the barcoding fragment of COI gene, and sequenced. The obtained data were analyzed on the novel mBRAVE platform, an online open‐access software and computing resource. The water samples were collected in 2 days by two people, and the laboratory analysis was completed in 5 days by one person. Overall, we detected the presence of 506 BINs identified as belonging to 435 taxa, representing at least 265 putative species. On average, only 5.4% of the taxa were shared among six replicates per site. Based on the observed diversity, three distinct clusters were detected and related to the geographic distribution of sites. There were some taxa shared between the habitats, with a substantial presence of terrestrial biota. Here we propose a new form of BioBlitz, where with noninvasive sampling effort combined with swift processing and straightforward online analyses, hundreds of species can be detected. Thus, using eDNA analysis of water is useful for rapid biodiversity surveys and valuable for educational purposes. We show that rapid eDNA surveys, combined with openly available services and software, can be used as an educational tool to raise awareness about the importance of biodiversity.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.urihttps://doi.org/10.1002/edn3.152en_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleAn urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNAen_US
dc.typeJournal Articleen_US
dc.identifier.eissn2637-4943
dc.identifier.journalEnvironmental DNAen_US
pubs.organisational-group/Natural History Museum
pubs.organisational-group/Natural History Museum/Science Group
pubs.organisational-group/Natural History Museum/Science Group/Functional groups
pubs.organisational-group/Natural History Museum/Science Group/Functional groups/Other Support
pubs.organisational-group/Natural History Museum/Science Group/Life Sciences
dc.embargoNot knownen_US
elements.import.authorHupało, Ken_US
elements.import.authorMajaneva, Men_US
elements.import.authorCzachur, MVen_US
elements.import.authorSire, Len_US
elements.import.authorMarquina, Den_US
elements.import.authorLijtmaer, DAen_US
elements.import.authorIvanov, Ven_US
elements.import.authorLeidenberger, Sen_US
elements.import.authorČiampor, Fen_US
elements.import.authorČiamporová‐Zaťovičová, Zen_US
elements.import.authorMendes, ISen_US
elements.import.authorDesiderato, Aen_US
elements.import.authorTopstad, Len_US
elements.import.authorMeganck, Ken_US
elements.import.authorHariz Z. A., Den_US
elements.import.authorKjærstad, Gen_US
elements.import.authorLin, Xen_US
elements.import.authorPrice, Ben_US
elements.import.authorStevens, Men_US
elements.import.authorEkrem, Ten_US
elements.import.authorDeiner, Ken_US
dc.description.nhm© 2020 The Authors. Environmental DNA published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The attached file is the published pdf.en_US
dc.subject.nhmaquatic habitatsen_US
dc.subject.nhmcytochrome c oxidase subunit I geneen_US
dc.subject.nhmenvironmental DNAen_US
dc.subject.nhmenvironmental educationen_US
dc.subject.nhmmBRAVEen_US
dc.subject.nhmmetabarcodingen_US
refterms.dateFOA2020-11-02T13:57:49Z


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