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dc.contributor.authorVanhove, Maarten PM
dc.contributor.authorBriscoe, Andrew G
dc.contributor.authorJorissen, Michiel WP
dc.contributor.authorLittlewood, T
dc.contributor.authorHuyse, Tine
dc.date.accessioned2020-11-19T09:41:15Z
dc.date.available2020-11-19T09:41:15Z
dc.date.issued2018-07-04
dc.date.submitted2020-11-19
dc.identifier.citationVanhove, M.P.M., Briscoe, A.G., Jorissen, M.W.P. et al. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae). BMC Genomics 19, 520 (2018). https://doi.org/10.1186/s12864-018-4893-5en_US
dc.identifier.issn1471-2164
dc.identifier.doi10.1186/s12864-018-4893-5
dc.identifier.urihttp://hdl.handle.net/10141/622872
dc.description.abstractBACKGROUND:Monogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective. RESULTS:Using Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus halli, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly 'primitive' African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines. CONCLUSIONS:The amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)en_US
dc.typeJournal Articleen_US
dc.identifier.eissn1471-2164
dc.identifier.journalBMC Genomicsen_US
dc.date.updated2020-11-19T08:59:19Z
dc.identifier.volume19en_US
dc.identifier.issue1en_US
dc.identifier.startpage520-en_US
dc.description.nhmOpen Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.subject.nhmCichlidaeen_US
dc.subject.nhmClariidaeen_US
dc.subject.nhmCichlidogyrusen_US
dc.subject.nhmGene orderen_US
dc.subject.nhmGyrodactylusen_US
dc.subject.nhmMacrogyrodactylusen_US
dc.subject.nhmMitogenomeen_US
dc.subject.nhmMonogeneaen_US
dc.subject.nhmMonopisthocotyleaen_US
dc.subject.nhmphylogenomicsen_US
refterms.dateFOA2020-11-19T09:41:16Z


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