Novel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hosts
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Authors
Cook, ShelleyChung, Betty Y-W
Bass, David
Moureau, Gregory
Tang, Shuoya
McAlister, Erica
Culverwell, CL
Glücksman, Edvard
Wang, Hui
Brown, T David K
Gould, Ernest A
Harbach, RE
de Lamballerie, Xavier
Firth, Andrew E
Issue date
2013-11-18Submitted date
2021-02-23Subject Terms
MosquitoesChironomids
Anopheles maculipennis
Anopheles detritus
RNA sequencing
Viruses
Metadata
Show full item recordAbstract
We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.Citation
Novel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hosts Cook S, Chung BYW, Bass D, Moureau G, Tang S, et al. (2013) Novel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hosts. PLOS ONE 8(11): e80720. https://doi.org/10.1371/journal.pone.0080720Publisher
Public Library of Science (PLoS)Journal
PLoS ONEType
Journal ArticleItem Description
© 2013 Cook et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.NHM Repository
ISSN
1932-6203EISSN
1932-6203ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0080720
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