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dc.contributor.authorCook, Shelley
dc.contributor.authorChung, Betty Y-W
dc.contributor.authorBass, David
dc.contributor.authorMoureau, Gregory
dc.contributor.authorTang, Shuoya
dc.contributor.authorMcAlister, Erica
dc.contributor.authorCulverwell, CL
dc.contributor.authorGlücksman, Edvard
dc.contributor.authorWang, Hui
dc.contributor.authorBrown, T David K
dc.contributor.authorGould, Ernest A
dc.contributor.authorHarbach, RE
dc.contributor.authorde Lamballerie, Xavier
dc.contributor.authorFirth, Andrew E
dc.date.accessioned2021-03-01T14:57:49Z
dc.date.available2021-03-01T14:57:49Z
dc.date.issued2013-11-18
dc.date.submitted2021-02-23
dc.identifier.citationNovel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hosts Cook S, Chung BYW, Bass D, Moureau G, Tang S, et al. (2013) Novel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hosts. PLOS ONE 8(11): e80720. https://doi.org/10.1371/journal.pone.0080720en_US
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0080720
dc.identifier.urihttp://hdl.handle.net/10141/622899
dc.descriptionTimes Cited: 0 0 Import from WoS only - CL not assigned grouping, NHM authors etc.en_US
dc.description.abstractWe investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleNovel Virus Discovery and Genome Reconstruction from Field RNA Samples Reveals Highly Divergent Viruses in Dipteran Hostsen_US
dc.typeJournal Articleen_US
dc.identifier.eissn1932-6203
dc.identifier.journalPLoS ONEen_US
dc.date.updated2021-02-23T11:03:02Z
dc.identifier.volume8en_US
dc.identifier.issue11en_US
dc.identifier.startpagee80720-e80720en_US
dc.description.nhm© 2013 Cook et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subject.nhmMosquitoesen_US
dc.subject.nhmChironomidsen_US
dc.subject.nhmAnopheles maculipennisen_US
dc.subject.nhmAnopheles detritusen_US
dc.subject.nhmRNA sequencingen_US
dc.subject.nhmVirusesen_US
refterms.dateFOA2021-03-01T14:57:49Z


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