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dc.contributor.authorKrämer, Jonas
dc.contributor.authorPeigneur, Steve
dc.contributor.authorTytgat, Jan
dc.contributor.authorJenner, Ronald
dc.contributor.authorvan Toor, Ronald
dc.contributor.authorPredel, Reinhard
dc.date.accessioned2022-06-17T13:34:51Z
dc.date.available2022-06-17T13:34:51Z
dc.date.issued2021-08-18
dc.date.submitted2021-06-30
dc.identifier.citationJonas Krämer, Steve Peigneur, Jan Tytgat, Ronald A. Jenner, Ronald van Toor, Reinhard Predel, A Pseudoscorpion's Promising Pinch: The venom of Chelifer cancroides contains a rich source of novel compounds, Toxicon, Volume 201, 2021, Pages 92-104, ISSN 0041-0101, https://doi.org/10.1016/j.toxicon.2021.08.012.en_US
dc.identifier.issn0041-0101
dc.identifier.doi10.1016/j.toxicon.2021.08.012
dc.identifier.urihttp://hdl.handle.net/10141/622981
dc.description.abstractWith pedipalps modified for venom injection, some pseudoscorpions possess a unique venom delivery system, which evolved independently from those of other arachnids like scorpions and spiders. Up to now, only a few studies have been focused on pseudoscorpion venom, which either identified a small fraction of venom compounds, or were based on solely transcriptomic approaches. Only one study addressed the bioactivity of pseudoscorpion venom. Here, we expand existing knowledge about pseudoscorpion venom by providing a comprehensive proteomic and transcriptomic analysis of the venom of Chelifer cancroides. We identified the first putative genuine toxins in the venom of C. cancroides and we showed that a large fraction of the venom comprises novel compounds. In addition, we tested the activity of the venom at specific ion channels for the first time. These tests demonstrate that the venom of C. cancroides causes inhibition of a voltage-gated insect potassium channel (Shaker IR) and modulates the inactivation process of voltage-gated sodium channels from Varroa destructor. For one of the smallest venomous animals ever studied, today's toolkits enabled a comprehensive venom analysis. This is demonstrated by allocating our identified venom compounds to more than half of the prominent ion signals in MALDI-TOF mass spectra of venom samples. The present study is a starting point for understanding the complex composition and activity of pseudoscorpion venom and provides a potential rich source of bioactive compounds useable for basic research and industrial application.en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA Pseudoscorpion's Promising Pinch: The venom of Chelifer cancroides contains a rich source of novel compoundsen_US
dc.typeJournal Articleen_US
dc.identifier.eissn1879-3150
dc.identifier.journalToxiconen_US
dc.date.updated2022-06-14T14:50:12Z
dc.identifier.volume201en_US
dc.identifier.startpage92-104en_US
elements.import.authorKrämer, Jonas
elements.import.authorPeigneur, Steve
elements.import.authorTytgat, Jan
elements.import.authorJenner, Ronald A
elements.import.authorvan Toor, Ronald
elements.import.authorPredel, Reinhard
dc.description.nhmCopyright: © The Authors. This document is the authors' submitted version of the journal article. You are advised to consult the published version if you wish to cite from it.en_US
dc.description.nhmNHM Repository
dc.subject.nhmMass spectrometryen_US
dc.subject.nhmtoxinsen_US
dc.subject.nhmelectrophysiologyen_US
dc.subject.nhmactivity testsen_US
dc.subject.nhmvenom extractionen_US
dc.subject.nhmproteomicsen_US
dc.subject.nhmtranscriptomicsen_US
refterms.dateFOA2022-06-17T13:34:52Z


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