Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system
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Authors
Richter, SandyHelm, Conrad
Meunier, Frederic A
Hering, Lars
Campbell, Lahcen I
Drukewitz, Stephan H
Undheim, Eivind AB
Jenner, Ronald
Schiavo, Giampietro
Bleidorn, Christoph
Issue date
2017-03-04Submitted date
2016-10-23Subject Terms
annelidaglyceridae
calcium channel
neurotoxin
GLTx
venom system
Metadata
Show full item recordAbstract
Background: We present the first molecular characterization of glycerotoxin (GLTx), a potent neurotoxin found in the venom of the bloodworm Glycera tridactyla (Glyceridae, Annelida). Within the animal kingdom, GLTx shows a unique mode of action as it can specifically up-regulate the activity of Ca<sub>v</sub>2.2 channels (N-type) in a reversible manner. The lack of sequence information has so far hampered a detailed understanding of its mode of action. Results: Our analyses reveal three ~3.8 kb GLTx full-length transcripts, show that GLTx represents a multigene family, and suggest it functions as a dimer. An integrative approach using transcriptomics, quantitative real-time PCR, in situ hybridization, and immunocytochemistry shows that GLTx is highly expressed exclusively in four pharyngeal lobes, a previously unrecognized part of the venom apparatus. Conclusions: Our results overturn a century old textbook view on the glycerid venom system, suggesting that it is anatomically and functionally much more complex than previously thought. The herein presented GLTx sequence information constitutes an important step towards the establishment of GLTx as a versatile tool to understand the mechanism of synaptic function, as well as the mode of action of this novel neurotoxin.Citation
Richter, S., Helm, C., Meunier, F.A. et al. Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system. BMC Evol Biol 17, 64 (2017). https://doi.org/10.1186/s12862-017-0904-4Publisher
Springer Science and Business Media LLCJournal
BMC Evolutionary BiologyType
Journal ArticleItem Description
Copyright © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.NHM Repository
ISSN
1471-2148EISSN
1471-2148ae974a485f413a2113503eed53cd6c53
10.1186/s12862-017-0904-4
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