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dc.contributor.authorArcher, John
dc.contributor.authorBarksby, Rebecca
dc.contributor.authorPennance, T
dc.contributor.authorRostron, Penelope
dc.contributor.authorBakar, Faki
dc.contributor.authorKnopp, Stefanie
dc.contributor.authorAllan, F
dc.contributor.authorKabole, Fatma
dc.contributor.authorAli, Said M
dc.contributor.authorAme, Shaali M
dc.contributor.authorRollinson, D
dc.contributor.authorWebster, BL
dc.date.accessioned2022-07-01T15:07:41Z
dc.date.available2022-07-01T15:07:41Z
dc.date.issued2020-09-11
dc.date.submitted2020-07-31
dc.identifier.citationArcher J, Barksby R, Pennance T, Rostron P, Bakar F, Knopp S, Allan F, Kabole F, Ali SM, Ame SM, Rollinson D, Webster BL. Analytical and Clinical Assessment of a Portable, Isothermal Recombinase Polymerase Amplification (RPA) Assay for the Molecular Diagnosis of Urogenital Schistosomiasis. Molecules. 2020; 25(18):4175. https://doi.org/10.3390/molecules25184175en_US
dc.identifier.issn1420-3049
dc.identifier.doi10.3390/molecules25184175
dc.identifier.urihttp://hdl.handle.net/10141/623009
dc.description.abstractAccurate diagnosis of urogenital schistosomiasis is crucial for disease surveillance and control. Routine diagnostic methods, however, lack sensitivity when assessing patients with low levels of infection still able to maintain pathogen transmission. Therefore, there is a need for highly sensitive diagnostic tools that can be used at the point-of-care in endemic areas. Recombinase polymerase amplification (RPA) is a rapid and sensitive diagnostic tool that has been used to diagnose several pathogens at the point-of-care. Here, the analytical performance of a previously developed RPA assay (RT-ShDra1-RPA) targeting the Schistosoma haematobium Dra1 genomic region was assessed using commercially synthesised S. haematobium Dra1 copies and laboratory-prepared samples spiked with S. haematobium eggs. Clinical performance was also assessed by comparing diagnostic outcomes with that of a reference diagnostic standard, urine-egg microscopy. The RT-ShDra1-RPA was able to detect 1 × 101 copies of commercially synthesised Dra1 DNA as well as one S. haematobium egg within laboratory-spiked ddH2O samples. When compared with urine-egg microscopy, the overall sensitivity and specificity of the RT-ShDra1-RPA assay was 93.7% (±88.7–96.9) and 100% (±69.1–100), respectively. Positive and negative predictive values were 100% (±97.5–100) and 50% (±27.2–72.8), respectively. The RT-ShDra1-RPA therefore shows promise as a rapid and highly sensitive diagnostic tool able to diagnose urogenital schistosomiasis at the point-of-care.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.rightsopenAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleAnalytical and Clinical Assessment of a Portable, Isothermal Recombinase Polymerase Amplification (RPA) Assay for the Molecular Diagnosis of Urogenital Schistosomiasisen_US
dc.typeJournal Articleen_US
dc.identifier.eissn1420-3049
dc.identifier.journalMoleculesen_US
dc.date.updated2022-06-17T16:04:05Z
dc.identifier.volume25en_US
dc.identifier.issue18en_US
dc.identifier.startpage4175-4175en_US
elements.import.authorArcher, John
elements.import.authorBarksby, Rebecca
elements.import.authorPennance, Tom
elements.import.authorRostron, Penelope
elements.import.authorBakar, Faki
elements.import.authorKnopp, Stefanie
elements.import.authorAllan, Fiona
elements.import.authorKabole, Fatma
elements.import.authorAli, Said M
elements.import.authorAme, Shaali M
elements.import.authorRollinson, David
elements.import.authorWebster, Bonnie L
dc.description.nhmCopyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). The attached file is the published version of the article.en_US
dc.subject.nhmUrogenital schistomiasisen_US
dc.subject.nhmSchistosoma haematobiumen_US
dc.subject.nhmpoint-of-careen_US
dc.subject.nhmdiagnosisen_US
dc.subject.nhmrecombinase polymerase amplification (RPA)en_US
dc.subject.nhmcontrolen_US
dc.subject.nhmsurveillanceen_US
dc.subject.nhmeliminationen_US
refterms.dateFOA2022-07-01T15:07:47Z


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